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Peripheral blood mononuclear cell (PBMC) suspensions will be prepared within 24 hours of blood collection by low-density gradient centrifugation via Hermle Z300 (K#55085010) to avoid erythrophagy-related activation of the monocytes. PBMCs will be frozen and stored at –80 °C using 10% dimethylsulfoxide, thereby allowing all samples to be analyzed in parallel. Plasma will also be stored at –80 °C.
JMIR Res Protoc 2025;14:e66439
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