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Assessment of the Effective Sensitivity of SARS-CoV-2 Sample Pooling Based on a Large-Scale Screening Experience: Retrospective Analysis

Assessment of the Effective Sensitivity of SARS-CoV-2 Sample Pooling Based on a Large-Scale Screening Experience: Retrospective Analysis

This work is a retrospective analysis of real-time polymerase chain reaction (RT-PCR) results for SARS-Co V-2 from 888,665 saliva samples using Dorfman pooling. From August 2020 to February 2022, 56,515 pools of varying sizes and 54,194 individual samples were tested, with 39,928 samples excluded for various reasons.

Jorge J Cabrera Alvargonzalez, Ana Larrañaga, Javier Martinez, Sonia Pérez Castro, Sonia Rey Cao, Carlos Daviña Nuñez, Víctor Del Campo Pérez, Carmen Duran Parrondo, Silvia Suarez Luque, Elena González Alonso, Alfredo José Silva Tojo, Jacobo Porteiro, Benito Regueiro

JMIR Public Health Surveill 2024;10:e54503

Molecular Detection of SARS-CoV-2 From Throat Swabs Performed With or Without Specimen Collection From the Tonsils: Protocol for a Multicenter Randomized Controlled Trial

Molecular Detection of SARS-CoV-2 From Throat Swabs Performed With or Without Specimen Collection From the Tonsils: Protocol for a Multicenter Randomized Controlled Trial

Individuals with or without symptoms of upper respiratory tract infection, aged 18 years or older, who visit Valby or Hillerød test center for reverse transcriptase–polymerase chain reaction (RT-PCR) testing for SARS-Co V-2 will be offered participation in the study. The exclusion criteria are individuals with a tracheostomy, laryngectomy, or prior oropharyngeal cancer surgery that would make the throat swab difficult.

Benedikte Hartvigsen, Kathrine Kronberg Jakobsen, Thomas Benfield, Niels Tobias Gredal, Annette Kjær Ersbøll, Mathias Waldemar Grønlund, Henning Bundgaard, Mikkel Porsborg Andersen, Nina Steenhard, Christian von Buchwald, Tobias Todsen

JMIR Res Protoc 2024;13:e47446

Assessing SARS-CoV-2 Testing Adherence in a University Town: Recurrent Event Modeling Analysis

Assessing SARS-CoV-2 Testing Adherence in a University Town: Recurrent Event Modeling Analysis

Polymerase chain reaction (PCR) testing accessibility was enhanced with the establishment of new testing locations offering voluntary and complimentary SARS-Co V-2 testing. HDT engaged over 200 public health ambassador students dedicated to advocating for healthy behaviors. Mass communication campaigns were promoted across diverse media platforms to encourage testing and the adoption of health-promoting behavior. The program provided incentives to promote health-promoting behaviors.

Yury E García, Alec J Schmidt, Leslie Solis, María L Daza-Torres, J Cricelio Montesinos-López, Brad H Pollock, Miriam Nuño

JMIR Public Health Surveill 2024;10:e48784

An Agreement of Antigen Tests on Oral Pharyngeal Swabs or Less Invasive Testing With Reverse Transcription Polymerase Chain Reaction for Detecting SARS-CoV-2 in Adults: Protocol for a Prospective Nationwide Observational Study

An Agreement of Antigen Tests on Oral Pharyngeal Swabs or Less Invasive Testing With Reverse Transcription Polymerase Chain Reaction for Detecting SARS-CoV-2 in Adults: Protocol for a Prospective Nationwide Observational Study

PCR and PCR-like technologies (nucleic acid amplification technologies [NAATs]) are considered the gold standard for detection of viral pathogens, but due to the extreme need for tests, the national RT-PCR testing capacity was unable to meet the overall testing demand. Consequently, RATs, which may be performed by non-health-care-trained individuals outside of health care facilities with results within minutes, were implemented on a large scale.

Uffe Vest Schneider, Jenny Dahl Knudsen, Anders Koch, Nikolai Søren Kirkby, Jan Gorm Lisby

JMIR Res Protoc 2022;11(5):e35706

Pool Testing as a Strategy for Prevention of SARS-CoV-2 Outbreaks in Schools: Protocol for a Feasibility Study

Pool Testing as a Strategy for Prevention of SARS-CoV-2 Outbreaks in Schools: Protocol for a Feasibility Study

Until now, we are aware of two studies successfully implementing a regular surveillance program for the monitoring of SARS-Co V-2 infections in schools, both of which involve collection of swabs by health care staff for real-time reverse transcription polymerase chain reaction (r RT-PCR) testing [33,34]. Despite massive expansion of testing capacities, however, regular testing and provision of rapid results for all school children would place a substantial logistical burden on schools.

Catherine M Sweeney-Reed, Doreen Wolff, Jakob Niggel, Michael Kabesch, Christian Apfelbacher

JMIR Res Protoc 2021;10(5):e28673

Interoperable Platform to Report Polymerase Chain Reaction SARS-CoV-2 Tests From Laboratories to the Chilean Government: Development and Implementation Study

Interoperable Platform to Report Polymerase Chain Reaction SARS-CoV-2 Tests From Laboratories to the Chilean Government: Development and Implementation Study

The polymerase chain reaction (PCR)–based tests are effective for diagnostic testing that looks for the SARS-Co V-2 virus’s genetic material, which causes COVID-19 [4]. As per the Centers for Disease Control and Prevention recommendations [4], the PCR test is the gold standard and accurate method for detecting, tracking, and studying COVID-19 [5]. The COVID-19 pandemic is especially challenging for laboratories tasked with rapid and reliable testing of an increased number of PCR tests [6].

Sergio Guinez Guinez-Molinos, José María Andrade, Alejandro Medina Negrete, Sonia Espinoza Vidal, Elvis Rios

JMIR Med Inform 2021;9(1):e25149

YouTube Videos Demonstrating the Nasopharyngeal Swab Technique for SARS-CoV-2 Specimen Collection: Content Analysis

YouTube Videos Demonstrating the Nasopharyngeal Swab Technique for SARS-CoV-2 Specimen Collection: Content Analysis

Qualitative real-time polymerase chain reaction (RT-PCR) of nasopharyngeal secretions is the gold standard for testing respiratory viruses, including SARS-Co V-2 [1]. However, major concerns have been raised regarding false-negative rates of RT-PCR tests in community testing locations [2]. An early retrospective review of community hospital testing performed in China reported a test sensitivity of only 71% [3].

Kyohei Itamura, Arthur Wu, Elisa Illing, Jonathan Ting, Thomas Higgins

JMIR Public Health Surveill 2021;7(1):e24220

Detection of SARS-CoV-2 RNA and Antibodies in Diverse Samples: Protocol to Validate the Sufficiency of Provider-Observed, Home-Collected Blood, Saliva, and Oropharyngeal Samples

Detection of SARS-CoV-2 RNA and Antibodies in Diverse Samples: Protocol to Validate the Sufficiency of Provider-Observed, Home-Collected Blood, Saliva, and Oropharyngeal Samples

We propose methods to validate multiple sample types for RNA-PCR (polymerase chain reaction) and for serology tests. Proposed specimen types and assays are depicted in Table 1. Specimen types and assays to be performed in an evaluation of diverse samples for SARS-Co V-2 (severe acute respiratory syndrome coronavirus 2) RNA and antibody testing. a Ig G: immunoglobulin G. b Ig M: immunoglobulin M. c Ig A: immunoglobulin A. Patients will be provided with printed instructions for collection (Figure 1).

Patrick Sean Sean Sullivan, Charles Sailey, Jodie Lynn Guest, Jeannette Guarner, Colleen Kelley, Aaron Julius Siegler, Mariah Valentine-Graves, Laura Gravens, Carlos del Rio, Travis Howard Sanchez

JMIR Public Health Surveill 2020;6(2):e19054