This cross-sectional study recruited 396 HCWs employed in public and private hospitals, dispensaries, pharmacies, and laboratories in Ibb City, the capital of Ibb Governorate located in the highlands of southwestern Yemen, between July 2022 and January 2023. A convenience sampling strategy was used for recruitment. Eligibility criteria for study inclusion required participants to be adults (age ≥18 years), employed in a health care setting for at least the 6 months preceding enrollment, unvaccinated against COVID-19, and to have provided written informed consent. The sole exclusion criterion was the previous receipt of 1 or more doses of any COVID-19 vaccine. To implement the convenience sampling, we visited various health care facilities during working hours. Eligible HCWs who were present, available, and willing to participate during the researchers’ visits were enrolled consecutively during the study period. For this, HCWs were visited at their workplaces during working hours and participated in structured interviews based on a predesigned questionnaire. Occupational and clinical data were collected from all participants during sample collection. HCWs were categorized according to the Yemen Medical Council’s classification, including physicians, nurses, dentists, pharmacists, physician assistants, and laboratory technicians.

Capillary whole-blood samples were obtained from all participants, and SARS-CoV-2 IgM and IgG antibodies were detected using a lateral flow immunoassay test according to the manufacturer’s instructions (Clungene, Shanghai International Holding Corp). Briefly, 5 µL of whole blood and 80 µL of sample buffer were applied. If antibodies against SARS-CoV-2 were present, they bound to the labeled antigens, forming antigen-antibody complexes that migrated to the test lines, where immobilized capture antibodies specific for SARS-CoV-2 antibodies were located. Clinical symptoms within the past 6 months were recorded using a standardized questionnaire.

Data analysis was performed using SPSS (version 25.0; IBM Corp). Chi-square tests were used to assess associations between categorical variables. Logistic regression analyses were conducted to calculate odds ratios (ORs) and 95% CIs. The prevalence of positive test results was analyzed in relation to sex, age groups, occupation types, and symptoms. Statistical significance was defined as P<.05 for all tests without correction for multiple testing.

The study received ethical approval from the Research Ethics Committee of Ibb University, Faculty of Medicine and Health Sciences (registration number IBBUNI.AC.YEM. 2022.35). Informed consent was obtained from all participants. Before analysis, participants’ data were deidentified using pseudonymization. Participants were not financially compensated for participation in the study.

We herein report the serostatus for SARS-CoV-2 in 396 unvaccinated HCWs in Ibb City in 2022-2023 to be 67%. Seropositivity was highest in individuals working in a hospital (194/268, 74%) as compared with laboratory (34/268, 50.7%) or pharmacy staff (12/268, 54.5%).

Since the first laboratory-confirmed case of COVID-19 was reported in April 2020 in Mukalla City [15], Yemeni HCWs have faced the pandemic amid numerous challenges, including a scarcity of PPE, the absence of vaccines in many cities, ongoing conflict, and various crises affecting the country.

During the early stages of the pandemic, studies worldwide reported seropositivity rates in unvaccinated HCWs ranging from 8% to more than 40%, with large differences between countries and hospitals [16-18]. After the first wave of COVID-19 in Yemen, between May and September 2020, 19.4% of asymptomatic HCWs at the Médecins Sans Frontières Trauma Center in Aden, Yemen, tested seropositive for SARS-CoV-2 antibodies [13]. Several factors have contributed to the lack of COVID-19 vaccines in many regions of Yemen, including the ongoing conflict that has severely disrupted the health care system and damaged infrastructure, hindering vaccine delivery to remote or conflict-affected areas; limited resources; and misinformation or lack of awareness about the importance of vaccination. During the conflict, Ibb was deemed safer, attracting migrants and increasing population density. This influx strained limited health care services, complicating COVID-19 control efforts.

From late 2022 to early 2023, many countries, including those in the Arab world, began to consider COVID-19 an endemic disease as infection rates stabilized and COVID-19 cases became more predictable with fewer severe outcomes [19]. The case fatality rate of confirmed infections gradually decreased in Taiz Governorate in southern Yemen from 27% in 2020 to 12.8% and 5.8% in 2021 and 2022, respectively [12]. In parallel, the seroprevalence of SARS-CoV-2 among HCWs from 9 hospitals in the Lahj and Al-Dhalea governorates of Yemen increased to 94.2% between June 2022 and September 2022, underscoring the transition to an endemic situation [14]. Consistent with our findings, a meta-analysis of COVID-19 seroprevalence conducted across 49 studies involving 127,480 HCWs indicated that antibody prevalence varied from 0% to 45.3%. Notably, one of the key factors associated with higher seroprevalence rates was the shortage of PPE [14]. In line with our study, the war-torn conditions and limited availability of PPE had a significant impact on the increase in antibody prevalence (56.5%) of COVID-19 among HCWs in Tigray, Ethiopia [14]. Although this study also included several professions, such as nurses, radiologists, dentists, laboratory personnel, pharmacists, respiratory therapists, and nutritionists, we here report a considerably lower seroprevalence in a comparable time frame with significant occupation-related differences. In our study, direct patient care as a physician assistant, dentist, nurse, or physician was associated with the highest odds of seropositivity.

Our findings align with other studies that identified higher odds of infection or seropositivity among health care staff who provided direct patient care in confined spaces, had prolonged or repeated face-to-face contact, or had contact with the environment or materials used by patients with COVID-19, which presumably contributed to the increased risk in these occupational groups [20-22]. The inadequate availability of PPE in hospitals may also have contributed to this risk, given the higher number of HCWs in hospitals compared with laboratories. Other studies [23-25] have found no significant correlation between occupation and infection, possibly reflecting differences in the availability and use of PPE and possible exposure from colleagues and household members.

In our study, seroprevalence of SARS-CoV-2 was highest among HCWs aged 40 years or older (33/40, 82.5%), compared with those younger than 40 years (235/356, 66%). This contrasts with studies from the Western countries, where higher seropositivity rates have been reported among younger HCWs, possibly due to increased exposure through social behaviors and patient contact, as well as differences in adherence to stringent protective measures [16,26].

Our analysis indicated significantly higher odds of SARS-CoV-2 seropositivity associated with typical symptoms such as fever, chills, headache, loss of taste, loss of smell, and sore throat. Other studies in HCWs have highlighted a strong association of loss of taste or smell with seropositivity [27-29]. In our study, loss of taste or smell was observed in 57.8% (155/268) of seropositive HCWs and associated with 2.3-fold odds. Notably, anosmia within the last 6 months was also reported by 45 (35%) seronegative HCWs, which is considerably higher than the self-reported olfactory loss (<15%) or objective smell dysfunction (<25%) in the general population [30]. This might, in part, be explained by false-negative results from the assays used, which represents a limitation of the study.

Several limitations warrant discussion. First, the recruitment strategy used may have introduced selection bias, potentially limiting the generalizability of our findings. Although a large sample size of 396 HCWs was enrolled, this sample may not be fully representative of all unvaccinated HCWs in Ibb, Yemen. Furthermore, the composition of the study sample might not accurately reflect the overall workforce in the specific health care facilities from which participants were drawn. This may affect the generalizability of the results to other regions or health care settings. In addition, this study relied on structured interviews and self-reported data for occupational information and COVID-19–related symptoms, which may have introduced recall bias or inaccuracies in reporting.

In summary, the observed seroprevalence of SARS-CoV-2 among HCWs in Ibb City, Yemen, highlights the ongoing vulnerability of HCWs, particularly those with close patient contact, in resource-limited and conflict-affected regions. These findings underscore the urgent need for sustained efforts to enhance infection control measures, including the provision of PPE and broader access to vaccines. Monitoring occupation-related risks and the evolving epidemiological landscape is crucial for informing future public health strategies.

This study reveals a high prevalence of SARS-CoV-2 antibodies among unvaccinated HCWs in Ibb City, indicating significant exposure to the virus during the pandemic. The analysis identified key risk factors, particularly occupation and workplace settings, with nurses and physician assistants having elevated odds of infection. These findings highlight the urgent need for targeted interventions to protect HCWs, including enhanced safety protocols and vaccination campaigns. Furthermore, the data suggest the necessity for ongoing surveillance and support for HCWs, especially in high-risk environments such as hospitals, to mitigate the spread of COVID-19 and ensure the safety of both health care providers and patients. Continued research is essential to understand the long-term implications of COVID-19 exposure among HCWs and to develop targeted interventions that can more effectively protect this critical workforce in similar settings worldwide.